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UltraClean® Fecal DNA Isolation Kit
Overview
For 30 minute isolation of genomic DNA from fecal samples.Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 12811-50 | $185.00 |
|
| 100 preps | 12811-100 | $368.00 |
|
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Features
- Rapidly purifies DNA from 250 mg to 1 gram in 30 minutes
- Yields PCR ready DNA up to 50 kb plus
- Simultaneous isolation of both host DNA and microbial DNA
- Proprietary Inhibitor Removal Solution eliminates humic acid PCR inhibitors
- Efficiently lyses microorganisms, spores and fungi
Description
The UltraClean® Fecal DNA Kit is a bead-beating method designed to isolate cellular, PCR quality genomic DNA from stool samples, dried fecal samples, droppings, forensic samples, and gut material. Simultaneous isolation of both host and microbial DNA is achieved. The kit also ensures removal of PCR inhibitors from the starting sample. Our Vortex Adapter is recommended for cell lysis. This fecal DNA kit has been used by numerous law enforcement agencies, zoologists and forensic scientists.
Sample Kit
Click to try a sample.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 20 µg per filter |
| Throughput | 1 - 24 samples |
| Time | 30 minutes |
| Starting Amount | From 250 mg to 1 gram |
| Equipment Required | Vortex and Vortex Adapter |
Storage Conditions
Store at room temperature for up to 2 years.Publications
- Crump, B.C., Koch, E.W Attached Bacterial Populations Shared by Four Species of Aquatic Angiosperms. Applied and Enviromental Microbiologu. 74(19): 5948-5957 (2008).
- Li, J., Santo Somingo, J.W., Lamendella, R., Edge, T., Hill, S. Phylogenetic Diversity and Molecular Detection of Bacteria in Gull Feces. Applied and Environmental Microbiology. 74(13): 3969-3976 (2008).
- Stauffer, S.H., Birkenheuer, A.J., Levy, M.G., Marr, H., Gookin, J.L. Evaluation of Four DNA Extraction Methods for the Detection of Tritrichomonas foetus in Feline Stool Specimens by Polymerase Chain Reaction. Journal of Veterinary Diagnostic Investigation 20: 639-641 (2008).
Kit Components
| Component | 12811-50 | 12811-100 |
|---|---|---|
| Solution S1 Solution S2 Solution S3 Solution S4 Solution S5 Fecal Bead Solution Fecal Dry Bead Tubes IRS Solution Spin Filters In Tubes Collection Tubes |
1 x 3.3 ml 1 x 14 ml 1 x 50 ml 1 x 16.5 ml 1 x 2.8 ml 1 x 30 ml 50 1 x 11 ml 50 150 |
1 x 7 ml 1 x 30 ml 1 x 100 ml 1 x 30 ml 1 x 6 ml 1 x 60 ml 100 1 x 22 ml 100 300 |
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