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PowerViral® Environmental RNA/DNA Isolation Kit

Overview

Isolate viral and bacterial total nucleic acids from waste water, stool, biosolids and gut material.
Order
Description Catalog # Price
50 preps 28000-50 $350.00    
50 preps, including Glass Bead Tubes 28000-BUNDLE $375.00    

Features

  • Optimized for challenging samples – Isolate viral and bacterial DNA and RNA from waste water, stool, biosolids and gut material
  • Inhibitor Removal Technology® - Eliminates inhibitory substances, including polysaccharides, lipids and heme for nucleic acids that are ready to use in downstream applications
  • Optional bead beating protoocol - Available with Glass Bead Tubes for optimal lysis of solid samples and bacterial cells and without bead tubes for isolation of viral nucleic acids from liquid samples

Description

The PowerViral® Environmental RNA/DNA Isolation Kit is designed for fast and easy purification of viral and bacterial total nucleic acids from samples high in PCR inhibitors; including waste water, stool, biosolids, and gut material. Patented Inhibitor Removal Technology® (IRT) ensures complete removal of the inhibitory substances often contained in these materials, such as undigested plant material in the gut or heme compounds from lysed red blood cells, abundant in stool. The result is DNA and RNA that is ready to use in the most demanding downstream applications. Nucleic acids are eluted in RNase-Free Water and ready for PCR, cDNA synthesis and RT-qPCR. 

Protocol Overview

For stool samples or samples with a solid matrix, lysis is achieved using 0.1 mm Glass Bead Tubes in combination with a strong chemical lysis buffer that ensures efficient extraction of tough microorganisms in the bead beating step. For virus extraction from water samples and samples that do not require dispersion, the bead beating step may be skipped and virus extracted through chemical lysis only. 

If lysis with Glass Bead Tubes is required, purchase Catalog # 28000-BUNDLE, which contains the PowerViral® Environmental RNA/DNA Isolation Kit plus 0.1mm Glass Bead Tubes. Otherwise, purchase Catalog # 28000-50 which is the PowerViral® Environmental RNA/DNA Isolation Kit alone.

If additional DNase treatment is required, we recommend the RTS DNase™ Kit, Catalog # 15200-50.

Sample Kit

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Research and Development

A.

B.

C.

Figure 1. Higher yields of viral nucleic acids isolated using the PowerViral® Environmental RNA/DNA Isolation Kit. Observed detection (log10copies/ml determined by qPCR) of A) adenovirus 2 (AdV); B) poliovirus type 1 (PV1); and C) Bacteriophage MS2 (MS2) extracted using MO BIO, Zymo, or Qiagen kits from spiked molecular grade water (extraction efficiency controls), biosolid extracts, fecal suspensions, and 1MDS surface water concentrates. Three samples (1, 2, and 3 on the horizontal axis) for each type of environmental sample matrix were spiked with each virus. To adjust for the difference between the three extraction kits in their loading and elution volumes, the concentrations of the original spiked viruses were calculated from the qPCR data and expressed as log10copies/ml. Only PowerViral® Environmental RNA/DNA Isolation Kit detected viral nucleic acids from every sample type. Data courtesy of B. Iker, University of Arizona, Tucson, AZ.

Figure 2.  Fewer inhibitors were detected in nucleic acid samples isolated using the PowerViral® Environmental RNA/DNA Isolation Kit. Assessment of the presence of qPCR inhibitors in the nucleic acid extracts from three separate extraction kits was performed via a qPCR inhibition test using an internal murine norovirus plasmid (pMNV) control (1.0×105 copies/PCR tube) in the presence of 2.5l of nucleic acid extract. Three samples (1, 2, and 3 on the horizontal axis) for each type of environmental sample matrix (biosolid extracts, fecal suspensions, and 1MDS surface water concentrates) were tested. Only PowerViral® Environmental RNA/DNA Isolation Kit demostrates better purification across all sample types. Data courtesy of B. Iker, University of Arizona, Tucson, AZ.

 

PowerViral® Environmental RNA/DNA Isolation Kit
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MSDS

 28000

Protocol

 28000

Specifications

 

Format Silica Spin Filter Tubes
Method Bead Beating (optional)
Starting Amount (liquid samples) 200 µl 
Starting Amount (solid samples) 0.25 g
Binding Capacity 40 µg
Throughput 1-24 sample
Time 45 minutes
Equipment Required

Vortex & Vortex Adapter

Centrifuge


Storage Conditions

Store at room temperature (15-30°C) for up to 2 years.

Publications

Kit Components

Component 28000-50 28000-BUNDLE
Solution PV1 32 ml 32 ml
Solution PV2 11 ml 11 ml
Solution PV3 32 ml 32 ml
Solution PV4 2 x 30 ml 2 x 30 ml
Solution PV5 30 ml 30 ml
Solution PV6 5.5 ml 5.5 ml
Spin Filters 50  50
2 ml Collection Tubes 200  200
2.2 ml Lysate Tubes 50  50
Glass Bead Tubes, 0.1 mm Not included 50