Search:

Products by Application

Environmental
Nucleic Acid Purification
Clinical
Public Health
Forensic

Products by Category

Genomic DNA Isolation
Dye Dots
DNA Clean-Up
Plasmid DNA Isolation
RNA Isolation
DNA-Free Reagents
Homogenization Equipment
Growth Media
Plastics & Spin Filters
Lab Supplies
Certified Water
Enzymes
Samples

Product Finder

   Product Finder:
     Search for the best MO BIO
     product for your application








                
Home / Enzymes
Print
Printable Version

KAPA SYBR® FAST Universal 2X qPCR Master Mix

Overview

SYBR® FAST qPCR Kits are supplied as a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR.
*Available for purchase to US direct customers only
Order
Description Catalog # Price
100 reactions x 20 µl (1 ml) 51230-100 $72.00    
500 reactions x 20 µl (5 ml) 51230-500 $315.00    
1000 reactions x 20 µl (2 x 5 ml) 51230-1000 $588.00    

Related Products

PowerPlant® DNA Isolation Kit
PowerMax® Soil DNA Isolation Kit
PowerWater® DNA Isolation Kit
PowerSoil® DNA Isolation Kit
PowerFood™ Microbial DNA Isolation Kit

Features

- KAPA SYBR® FAST qPCR Kits are ideally suited for:

- Gene expression analysis

- Low copy gene detection

- Microarray validation

- Gene knockdown validation

- High-throughput qPCR

- ChIP

- Next-generation DNA sequencing library quantification

Description

KAPA SYBR® FAST qPCR Kits are designed for the highest performance in real-time PCR.

The kits contain a novel DNA polymerase engineered through a process of molecular evolution - the result is a unique enzyme, specifically evolved for qPCR using SYBR® Green I dye chemistry.

The KAPA SYBR® DNA Polymerase was engineered to perform optimally in stringent real-time qPCR reaction conditions, exhibiting dramatic improvements to signal-to-noise ratio (fluorescence), cycle threshold (Ct), linearity, and sensitivity.

The KAPA SYBR® DNA Polymerase and proprietary buffer system enhances the amplification efficiency of difficult templates, including both GC-rich and AT-rich templates.

SYBR® FAST qPCR Kits are supplied as a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The KAPA SYBR® FAST qPCR Kit is supplied as a 2x Master Mix with integrated antibody-mediated hot start, SYBR® Green I fluorescent dye, MgCl2, dNTPs, and stabilizers. 

KAPA SYBR® FAST qPCR Kits are intended for use on all real-time instruments and protocols.

* These enzymes are for research only.

 

Sample Kit

Click here to try a sample

KAPA SYBR® FAST Universal 2X qPCR Master Mix
Click over image to enlarge

Specifications

Cat # 51230-100: For 100 x 20 µl reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µl each).

Cat # 51230-500: For 500 x 20 µl reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µl each).

Cat # 51230-1000: For 1000 x 20 µl reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µl each). 

Disclaimer: MO BIO Laboratories, Inc. is an authorized distributor of  Kapa Biosystems.

 

* PLEASE NOTE FOR ALL PCR ENZYME SHIPPING, THERE WILL BE A $100.00 SHIPPING FEE FOR OVERNIGHT SERVICE AND PACKAGING MATERIALS.  OTHER MATERIALS IN YOUR ORDER WILL BE SHIPPED SEPARATELY, POSSIBLY ON DIFFERENT DAYS AND OUR REGULAR SHIPPING CHARGES WILL APPLY.

 

Storage Conditions

Store enzyme at -20°C for up to 1 year.

Customer Comments

"WOW! I tried your KAPA polymerase against my Bio-Rad iQ SYBR Green Supermix and I got much, much better results!

My fluorescent signals (SYBR) were ten times as strong, and, most importantly, the KAPA enzyme amplified a sample that has been absolutely flat-line inhibited until now!

I always run MP analyses, and they look better, too. I will be switching to your enzyme immediately; thanks for the advice!"

Rick Fowler,Western Kentucky University