KAPA2G Robust HotStart ReadyMix

Overview

Consolidate PCR protocols and increase success rates with 2G Robust HotStart DNA Polymerase and ReadyMix
*Available for purchase to US direct customers only

Order

Description	Catalog #	Price
100 reactions	51240-100	$90.00
500 reactions	51240-500	$342.00

Features

- Amplification from templates with a high GC- or AT content.

- Consolidation of PCR protocols and reaction conditions while improving success rates.

- Templates containing common PCR inhibitors (e.g. salts, urea, SDS and ethanol).

- Amplification from crude samples, e.g. buccal swabs, cultured mammalian, yeast or bacterial cells (Colony PCR).

- Optimization of low yield or low specificity assays.

Description

The KAPA2G Robust DNA Polymerase is a highly robust and versatile second-generation enzyme derived through a process of molecular evolution.

The novel amino acid mutations in KAPA2G Robust DNA Polymerase offer higher processivity and specific activity, which translates to robust performance across a wide range of GC- and AT-rich templates and amplicons, as well as improved tolerance to common PCR inhibitors.

In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency. KAPA2G Robust Kits supplied with KAPA2G Buffer A and KAPA2G Buffer B and the proprietary additive, KAPA Enhancer 1, offer extended optimization options for diverse and difficult templates.

Kits also contain KAPA2G GC Buffer, a novel buffer formulated specifically for GC-rich templates and amplicons. Like wild-type Taq, KAPA2G Robust DNA Polymerase has 5’-3’ polymerase and exonuclease activities, but no 3’-5’ exonuclease (proofreading activity). The fidelity of KAPA2G Robust DNA Polymerase is similar to that of wild-type Taq.

* These enzymes are for research only.

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Specifications

Cat # 51240-100: For 100 x 25 µl reactions. Convenient 2X master mix containing KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.0 mM. Just add template and primers.

Cat # 51240-500: For 500 x 25 µl reactions. Convenient 2X master mix containing KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.0 mM. Just add template and primers.

Disclaimer: MO BIO Laboratories, Inc. is an authorized distributor of Kapa Biosystems.

* PLEASE NOTE FOR ALL PCR ENZYME SHIPPING, THERE WILL BE A $100.00 SHIPPING FEE FOR OVERNIGHT SERVICE AND PACKAGING MATERIALS. OTHER MATERIALS IN YOUR ORDER WILL BE SHIPPED SEPARATELY, POSSIBLY ON DIFFERENT DAYS AND OUR REGULAR SHIPPING CHARGES WILL APPLY.

Storage Conditions

Store enzyme at -20°C for up to 1 year.