KAPA2G FAST HotStart

Overview

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Features

- Extension times as low as 1 sec per kilobase

- Outstanding performance when compared to conventional hot start Taq polymerase

- 20 - 70% reductions in total PCR time

- No requirement for specialist PCR consumables or instrumentation.

Description

KAPA2G Fast DNA Polymerase, a second-generation enzyme derived through a process of molecular evolution.

KAPA2G Fast DNA Polymerase was specifically engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step.

This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency.

KAPA2G Fast HotStart is particularly well suited for high throughput Fast PCR, offering total reaction times 20 - 70% shorter than conventional PCR assays performed with wild-type Taq polymerase or hot start formulations thereof. In addition, KAPA2G Fast HotStart has been shown to achieve higher yields and sensitivity than competitor enzymes across a wide range of amplicon types. No specialized PCR consumables or thermocyclers are required.

 * These enzymes are for research only.

Sample Kit

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Specifications

Cat # 51260-100: 100 units, 5U/µl. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1 conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).

Cat # 51260-250: 250 units, 5U/µl. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1 conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).

Cat # 51260-500: 500 units, 5U/µl. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1 conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).

Cat # 51270-100: 100 x 25µl rxns. Supplied with KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM.

Cat # 51270-500: 500 x 25µl rxns. Supplied with KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM.

Disclaimer: MO BIO Laboratories, Inc. is an authorized distributor of Kapa Biosystems.

* PLEASE NOTE FOR ALL PCR ENZYME SHIPPING, THERE WILL BE A $100.00 SHIPPING FEE FOR OVERNIGHT SERVICE AND PACKAGING MATERIALS. OTHER MATERIALS IN YOUR ORDER WILL BE SHIPPED SEPARATELY, POSSIBLY ON DIFFERENT DAYS AND OUR REGULAR SHIPPING CHARGES WILL APPLY.

Storage Conditions

Customer Comments

" This shaved 52.5 minutes off of our normal thermal protocol, plus additional time for the heating/cooling.