KAPA Long Range HotStart DNA Polymerase with dNTPs

Overview

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Features

- PCR amplification of long targets up to 20 kb and/or PCR using low amounts of template DNA

- Standard short- and mid-range PCR amplification

- Production of PCR products to be used for ligation into 3’-T-overhang cloning vectors

- Targeted sample enrichment for next-generation DNA sequencing.

Description

The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability.

This two-enzyme system is designed specifically to support long range and/or sensitive PCR. KAPA Long Range DNA Polymerase possesses 3x – 4x higher fidelity than Taq polymerase. The majority of PCR products from a KAPA Long Range HotStart DNA Polymerase reaction have a single deoxyadenosine added to the 3’ ends of the amplicon and can be used for ligation into single 3’-T -overhang cloning sites.

Improve the specificity during prolonged benchtop setup or liquid handling with the HotStart Technology.

*These enzymes are for research only

Specifications

Cat # 51280-100: 100 units, 5U/µl. Supplied with 5X Long Range Buffer A (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM).

Cat # 51280-250: 250 units, 5U/µl. Supplied with 5X Long Range Buffer A (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM).

Cat # 51280-500: 500 units, 5U/µl. Supplied with 5X Long Range Buffer A (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM).

Disclaimer: MO BIO Laboratories, Inc. is an authorized distributor of Kapa Biosystems.

* PLEASE NOTE FOR ALL PCR ENZYME SHIPPING, THERE WILL BE A $100.00 SHIPPING FEE FOR OVERNIGHT SERVICE AND PACKAGING MATERIALS.  OTHER MATERIALS IN YOUR ORDER WILL BE SHIPPED SEPARATELY, POSSIBLY ON DIFFERENT DAYS AND OUR REGULAR SHIPPING CHARGES WILL APPLY.

Storage Conditions