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KAPA HiFi Hotstart Ready Mix

Overview

The increased processivity, strong proofreading activity, and optimized buffer system of the engineered HiFi DNA Polymerase results in superior accuracy for high fidelity PCR applications.
*Available for purchase to US direct customers only
Order
Description Catalog # Price
100 reactions (25 µl reactions) 51250-100 $132.00    
500 reactions (25 µl reactions) 51250-500 $551.00    

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PowerMax® Soil DNA Isolation Kit
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PowerPlant® DNA Isolation Kit

Features

  • Site-directed mutagenesis
  • Sequencing
  • Protein expression
  • Next-generation DNA resequencing

Description

KAPA HiFi DNA Polymerase is a novel proofreading polymerase that was engineered to have high processivity, exhibiting significant improvements in yield, sensitivity, speed, target length, and ability to amplify difficult templates (e.g GC-rich) when compared to wild-type polymerases.

The HotStart formulation of the enzyme increases reaction efficiency and sensitivity by eliminating spurious amplification products resulting from non-specific priming events during reaction setup and initiation. KAPA HiFi PCR Kits include two buffers for optimal performance with difficult templates. Both buffers contain magnesium chloride allowing for convenient setup and robustness.

 

* These enzymes are for research only.

 

 

KAPA HiFi Hotstart Ready Mix
Click over image to enlarge

Specifications

Cat # 51250-100: 100 x 25 µl reactions. Convenient 2X master mix containing KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.0 mM. Just add template and primers.

 Cat # 51250-500: 500 x 25 µl reactions. Convenient 2X master mix containing KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.0 mM. Just add template and primers.

 Disclaimer: MO BIO Laboratories, Inc. is an authorized distributor of Kapa Biosystems.

 

 

 * PLEASE NOTE FOR ALL PCR ENZYME SHIPPING, THERE WILL BE A $100.00 SHIPPING FEE FOR OVERNIGHT SERVICE AND PACKAGING MATERIALS.  OTHER MATERIALS IN YOUR ORDER WILL BE SHIPPED SEPARATELY ON A DIFFERENT DAY AND OUR REGULAR SHIPPING CHARGES WILL APPLY.

 

Storage Conditions

Store enzyme at -20°C for up to 1 year.