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UltraClean® Endotoxin-Free Maxi Plasmid Prep Kit
Overview
For isolation of up to 1 mg of endotoxin-free UltraClean® plasmid DNAOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 10 preps | 12611-10 | $263.00 |
|
Related Products
UltraClean® Endotoxin Removal KitUltraClean® Endotoxin-Free Mini Plasmid Prep Kit
UltraClean® Endotoxin Removal Kit Components
Features
- Results in high quality plasmid DNA containing less then 0.1 EU/µg in less time
- Produces DNA suitable for transfection and injection
- Utilizes a simple silica spin filter for DNA yields equal to anion exchange columns
- Rapid protocol with no additional affinity columns required to remove lipopolysaccharides
Description
The UltraClean® Endotoxin-Free Maxi Plasmid Prep Kit* integrates an efficient endotoxin removal step into the plasmid purification procedure to make it the fastest endotoxin-free maxi prep method available. Purified, endotoxin-free DNA is suitable for transfection and injection.
No additional affinity columns to remove lipopolysaccharides are required. Cells are lysed with an improved set of alkaline lysis reagents. Endotoxins are selectively precipitated in a specialized buffer. The plasmid DNA is bound to a silica spin filter, washed once and released into endotoxin-free water (provided). The plasmid DNA is concentrated enough to be used directly in transfection experiments.
Endotoxins, also known as lipopolysaccharides, are cell membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. Endotoxins represent a non-controllable variable in transfection experiment setup, making results difficult to compare and interpret.
* DNA isolated with this kit should be used for research purposes only.
Research and Development
Comparison of US price per prep between MO BIO and two
other major suppliers of Endotoxin-Free Plasmid Isolation Kits.
supercoiled, transfection quality,
endotoxin-free plasmid DNA.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | 50 ml Silica Spin Filter Tubes |
|---|---|
| Method | Alkaline Lysis/Endo Removal |
| Binding Capacity | Up to 1 mg per filter |
| Throughput | 1 - 4 samples |
| Time | 90 minutes |
| Endotoxin Level | <0.1 EU/µg |
Storage Conditions
Store at room temperature for up to 2 yearsKit Components
| Component | 12611-10 |
|---|---|
| Solution EF1 Solution EF2 Solution EF3 Solution EF4 Solution EF5 Solution EF6 Solution EF7 Spin Filters In Tubes 50 ml Collection Tubes |
1 x 27.5 ml 1 x 55 ml 1 x 82.5 ml 1 x 33 ml 1 x 165 ml 1 x 75 ml 1 x 30 ml 10 60 |
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Individual kit components that can be purchased separately.
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