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DNase Detection Kit
Overview
For sensitive detection of DNase contamination.Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 1 Test Kit (4 samples) | 12093-1 | $125.00 |
|
| 2 Test Kit (8 samples) | 12093-2 | $216.00 |
|
| 5 Test Kit (20 samples) | 12093-5 | $509.00 |
|
Features
* DUE TO THE TEMPERATURE SENSITIVE NATURE OF THIS PRODUCT, WE ARE UNABLE TO SHIP OUTSIDE THE US.
- Rapid detection protocol takes less than 1.5 hours.
- Procedure complete in less than 1.5 hours.
- Test can detect as low as 10-7 Kunitz units/µl.
- User friendly protocol is as simple as performing an enzymatic digest and running an agarose gel.
- Kit is certified DNase-Free.
- All tubes and reagents required are included in kit and ready to use including individually wrapped pipet tips.
Description
The DNase Detection Kit* is designed to determine if DNase contamination is present in sample solutions. In the procedure, DNA is incubated with a test samples and is then run on an agarose gel for analysis. The DNase Detection Kit is capable of detecting as low as 10-7 Kunitz units/µl and is ideal for screening PCR buffers and reagents prior to amplification experiments. Shipped on dry ice. Must be kept frozen before use.
Up to four samples can be tested simultaneously with this kit. DNase activity can be quantified by comparison to the reference standards included. The kit is ideal for early detection of DNase activity in reagent solutions that will be used for sensitive DNA based experiments.
The procedure is to mix 1 µl of the test sample with a DNA Ladder standard provided. The sample is incubated at 37°C for 1 hour. After incubation, the sample is loaded onto a native agarose gel along with four control standards included with the kit. Results are interpreted from the gel analysis.
*For sale in the US only.
| 1 | 2 | 3 | 4 | 5 | 6 |
of DNA degradation as compared to complete
degradation by the positive control (lane 6).
- Lane 1: Test sample #1
Lane 2: Test sample #2
Lane 3: Test sample #3
Lane 4: Test sample #4
Lane 5: Negative control
Lane 6: Positive control
Click over image to enlarge
MSDS
Protocol
Specifications
The DNase Detection Kit is capable of detecting as low as 10-7 Kunitz units/µl. For clarity, 1 unit of DNase is commonly defined as the amount of enzyme that produces an increase of 1.0 A260 unit at 37°C when DNA is hydrolyzed to acid soluble oligonucleotides.
One Kunitz unit is equal to 50 DNase units. The increase of 1.0 A260 unit represents the loss of
50 µg/ml (0.05 µg/µl) or 1/50 Kunitz unit.
* PLEASE NOTE FOR ALL DNASE & RNASE DETECTION KITS, THERE WILL BE A $75.00 HANDLING FEE IN ADDITION TO THE OVERNIGHT SHIPPING CHARGE. THIS WILL BE DETERMINED BASED ON YOUR DOMESTIC SHIPPING ADDRESS WITHIN THE CONTINENTAL UNITED STATES. DUE TO THE TEMPERATURE SENSITIVE NATURE OF THIS PRODUCT, IT HAS TO SHIP NEXT DAY AIR. OTHER MATERIALS IN YOUR ORDER WILL BE SHIPPED SEPARATELY AND OUR REGULAR SHIPPING CHARGES WILL APPLY.
Storage Conditions
Store kit at -20°C to -70°C for up to 6 months.
Long term storage is recommended at -70°C.
DNase Detection Kit is shipped on dry ice.
Kit Components
| Component | 12093-1 | 12093-2 | 12093-5 |
|---|---|---|---|
| Standard A Standard B Control C Control D Control E Gel Dye Pipet Tips |
4 x 10 µl 1 x 10 µl 1 x 12 µl 1 x 12 µl 1 x 12 µl 1 x 20 µl 20 |
8 x 10 µl 2 x 10 µl 2 x 12 µl 2 x 12 µl 2 x 12 µl 2 x 20 µl 40 |
20 x 10 µl 5 x 10 µl 5 x 12 µl 5 x 12 µl 5 x 12 µl 5 x 20 µl 100 |
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