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UltraClean® PCR Clean-Up Kit
Overview
For DNA clean up from PCR, sequencing and enzyme reactions.Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 12500-50 | $79.00 |
|
| 100 preps | 12500-100 | $138.00 |
|
| 250 preps | 12500-250 | $330.00 |
|
Related Products
UltraClean® 15 DNA Purification KitUltraClean® -htp 96 Well PCR Clean-Up Kit
PowerClean® DNA Clean-Up Kit
UltraClean® GelSpin® DNA Extraction Kit
Features
- Yields exceptionally clean DNA in just 3 minutes
- Purifies DNA 60 bp to 10 kb with complete removal of primer-dimers up to 50 bases
- Easy-to-use silica spin filter format removes PCR primers, enzymes, labels and buffers
Description
The UltraClean® PCR Clean-Up Kit is designed to purify PCR products directly from a PCR or enzyme reaction. If you sequence your PCR reactions or have applications where efficient removal of PCR primers is critical, this kit is your solution.
All reagents are optimized to remove primers, dNTPs and reaction components while purifying PCR reaction products in the size range of 60 bp - 10 kb. For products larger than 10 kb, run the reaction on an agarose gel and isolate the large DNA using a gel reaction on an agarose gel and isolate the large DNA using a gel extraction kit like the UltraClean® 15 DNA Purification Kit or the UltraClean® GelSpin® DNA Extraction Kit.
NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System!
Sample Kit
Click to try a sample.
Research and Development
100% of the unwanted PCR primers
| -400 bp PCR product |
| -20 bp PCR primers |
Lane 1: Purification with the UltraClean® PCR Clean-Up Kit.
Lane 2: Purification using another quick kit.
Lane 3: PCR reaction before purification.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| DNA Size Range | 60 bp - 10 kb |
| Binding Capacity | Up to 20 µg DNA |
| Throughput | 1 - 24 samples |
| Time | 3 minutes |
| Equipment Required | Microcentrifuge |
100 bp 300 bp 10 kb |
95% 100% 95% |
Storage Conditions
Store at room temperature for up to 2 years
Publications
- Chapman, T.W., Marando, L., Oorebeek, M., and Kleindorfer, S. Genetic Structure in Ixodid Ticks from Kangaroo Island and the South Australia Mainland. Australian Journal of Entomology. 48: 40-46 (2009).
- Gozalbo-Lopez, B., Andrade, P., Terrados, G., de Andres, B., Serrano, N., Cortegano, I., Palacios, B., Bernad, A., Blanco, L., Marcos, M.A.R., and Gaspar, M.L. A Role for DNA Polymerase µ in the Emerging DJH Rearrangements of the Postgastrulation Mouse Embryo. Molecular and Cellular Biology. 29(5): 1266-1275 (2009).
- Harrington, M.G., Biffin, E., and Gadek, P.A. Comparative Study of the Evolution of Nuclear Ribosomal Spacers Incorporating Secondary Structure Analyzes within Dodonaeoideae, Hippocastanoideae and Xanthoceroidaea (Sapindaceae). Molecular Phylogenetics and Evolution. 50(2): 364-375 (2009).
Kit Components
| Component | 12500-50 | 12500-100 | 12500-250 |
|---|---|---|---|
| SpinBIND SpinClean Elution Buffer Collection Tubes Spin Filters In Tubes |
1 x 28 ml 1 x 16.5 ml 1 x 3 ml 50 50 |
1 x 55 ml 1 x 30 ml 1 x 6 ml 100 100 |
1 x 144 ml 3 x 30 ml 1 x 15 ml 250 250 |
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