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UltraClean® PCR Clean-Up Kit

Overview

For DNA clean up from PCR, sequencing and enzyme reactions.

On sale now! Offer valid for web orders only through 9/30/14.
Order
Description Catalog # Price
50 preps 12500-50 $85.00 $68.00
You Save: $17.00 N/A
   
100 preps 12500-100 $148.00 $118.00
You Save: $30.00 N/A
   
250 preps 12500-250 $354.00 $283.00
You Save: $71.00 N/A
   

Features

  • Yields exceptionally clean DNA in just 3 minutes
  •  Purifies DNA 60 bp to 10 kb with complete removal of primer-dimers up to 50 bases
  •  Easy-to-use silica spin filter format removes PCR primers, enzymes, labels and buffers

Description

The UltraClean® PCR Clean-Up Kit is designed to purify PCR products directly from a PCR or enzyme reaction. If you sequence your PCR reactions or have applications where efficient removal of PCR primers is critical, this kit is your solution.

All reagents are optimized to remove primers, dNTPs and reaction components while purifying PCR reaction products in the size range of 60 bp - 10 kb. For products larger than 10 kb, run the reaction on an agarose gel and isolate the large DNA using a gel reaction on an agarose gel and isolate the large DNA using a gel extraction kit like the UltraClean® 15 DNA Purification Kit or the UltraClean® GelSpin® DNA Extraction Kit.

Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System! 

Sample Kit

Click to try a sample.

Research and Development

The UltraClean® PCR Clean-Up Kit removes
100% of the unwanted PCR primers
 
 
-400 bp PCR product
 
-20 bp PCR primers
 


Lane 1: Purification with the UltraClean® PCR Clean-Up Kit.
Lane 2: Purification using another quick kit.
Lane 3: PCR reaction before purification.

 

 
UltraClean® PCR Clean-Up Kit
Click over image to enlarge

MSDS

12500

Protocol

12500

Specifications

Format Silica Spin Filter Tubes
DNA Size Range 60 bp - 10 kb
Binding Capacity Up to 20 µg DNA
Throughput 1 - 24 samples
Time 3 minutes
Equipment Required Microcentrifuge

 

Size vs. Recovery
DNA Size
Recovery
60 bp
100 bp
300 bp
10 kb
85%
95%
100%
95%

Storage Conditions

Store at room temperature for up to 2 years

Publications

Kit Components

Component 12500-50 12500-100 12500-250
SpinBIND
SpinClean™

Elution Buffer
Collection Tubes
Spin Filters
In Tubes
1 x 28 ml
1 x 16.5 ml
1 x 3 ml
50
50
 
1 x 55 ml
1 x 30 ml
1 x 6 ml
100
100
 
1 x 144 ml
3 x 30 ml
1 x 15 ml
250
250