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UltraClean® -htp 96 Well PCR Clean-Up Kit
Overview
For DNA clean up from PCR, sequencing and enzyme reactions in a convenient 96 well formatOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 4 x 96 preps | 12596-4 | $570.00 |
|
| 12 x 96 preps | 12596-12 | $1,537.00 |
|
Features
- Yields exceptionally clean DNA in just 15 minutes
- Purifies DNA 60 bp to 10 kb with complete removal of primer-dimers up to 50 bases
- Easy-to-use silica spin filter plate format removes PCR primers, enzymes, labels and buffers
Description
The UltraClean®-htp 96 Well PCR Clean-Up Kit is designed to purify PCR products directly from a PCR or enzyme reaction. If you sequence your PCR reactions or have applications where efficient removal of PCR primers is critical, this kit is your solution.
All reagents are optimized to remove primers, dNTPs and reaction components while purifying PCR reaction products in the size range of 60 bp - 10 kb. For products larger than 10 kb, run the reaction on an agarose gel and isolate the large DNA using a gel extraction kit like the UltraClean® 15 DNA Purification Kit or the UltraClean® GelSpin® DNA Extraction Kit.
Research and Development
Consistent Recovery Yields and Primer Removal with the UltraClean®-htp 96 Well PCR Clean-Up Kit
| -400 bp PCR product -20 bp PCR primers |
Lane 9: 50% of a PCR reaction before purification.
Lane 10: 100% of a PCR reaction before purification.
Analysis displayed on 1.2% TAE agarose gel.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Plate |
|---|---|
| DNA Size Range | 60 bp - 10 kb |
| Binding Capacity | Up to 20 µg DNA |
| Throughput | 96 samples |
| Time | 15 minutes |
| Equipment Required | Large Centrifuge |
Storage Conditions
Store at room temperature for up to 2 years.Publications
- Wolfe, B.E., Mummey, D.L., Rillig, M.C., Klironomos, J.N. Small-Scale Spatial Heterogeneity Of Arbuscular Mycorrhizal Fungal Abundance And Community Composition In A Wetland Plant Community. Mycorrhiza. 17(3): 175-183 (2007).
- Hayashi, H., Takahashi, R., Nishi, T., Sakamoto, M., and Benno, Y. Molecular Analysis of Jejunal, Ileal, Caecal and Retro-sigmoidal Human Colonic Microbiota Using 16S rRNA Gene Libraries and Terminal Restriction Fragment Length Polymorphism. Journal of Medical Microbiology. 54: 1093-1101 (2005).
- Wegley, L., Yu, Y., Breitbart, M., Casa, V., Kline, C.I., Rohwer, F. Coral-associated Archaea. Marine Ecology Progrss Series. 273: 89-96 (2004).
Kit Components
| Component | 12596-4 | 12596-12 |
|---|---|---|
| SpinBind SpinCLEAN Buffer Elution Buffer Spin Filter Plates 0.5 ml Collection Plates Sealing Tape Centrifuge Tape Microplate Elution Sealing Mat |
211 ml 4 x 30 ml 1 x 43 ml 4 12 4 12 4 4 |
633 ml 12 x 30 ml 1 x 129 ml 12 36 12 36 12 12 |