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UltraClean® GelSpin® DNA Extraction Kit
Overview
For fast and easy 5 minute DNA extraction from gelsOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 12400-50 | $84.00 |
|
| 100 preps | 12400-100 | $146.00 |
|
| 250 preps | 12400-250 | $353.00 |
|
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Features
- Purifies DNA from agarose gels in as little as 5 minutes.
- Utilizes a simple method to remove ethidium bromide, linkers and labels from TAE or TBE gels.
- Simply melt, bind, wash and collect DNA.
Description
The UltraClean® GelSpin® DNA Extraction Kit utilizes a silica-based spin filter membrane to isolate DNA from agarose gels. After electrophoresis, the desired DNA band is cut from the agarose gel and placed directly in the spin filter column. The kit uses a very simple technique to melt the gel, bind the DNA, wash it and recover it. Popular uses include purification from gels, restriction digests, sequencing reactions (dye removal), radioactive and non-radioactive labeling reactions. The kit also removes all gel stains like ethidium bromide, SYBR® Green and xylene cyanol. The DNA is immediately ready to use for restriction digest, ligation, labeling or PCR.
Sample Kit
Click to try a sample.
Research and Development
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 |
from a 100 bp DNA ladder (lane 13) with the
UltraClean® GelSpin® DNA Extraction Kit.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| DNA Size Range | 60 bp - 50 kb |
| Binding Capacity | 20 µg DNA |
| Throughput | 1 - 24 samples |
| Time | 5 minutes |
| Size vs. Recovery | |
|---|---|
| DNA Size | Recovery |
| 60 bp 100 bp 300 bp 500 bp 10 kb 50 kb |
75% 80% 100% 95% 90% 80% |
Storage Conditions
Store at room temperature for up to 2 years.
Publications
- Gozalbo-Lopez, B., Andrade, P., Terrados, G., de Andres, B., Serrano, N., Cortegano, I., Palacios, B., Bernad, A., Blanco, L., Marcos, M.A.R., and Gaspar, M.L. A Role for DNA Polymerase µ in the Emerging DJH Rearrangements of the Postgastrulation Mouse Embryo. Molecular and Cellular Biology. 29(5): 1266-1275 (2009).
- Wang., X., Moni, P.T., Saint, C.P., and Jin, B. Biocehmical Kinetics of Fermentative Hydrogen Production by Clostridium butyricum W5. International Journal of Hydrogen Energy. 34(2): 791-798 (2009).
- Chauhan, A., and Williams, H.N. Biostimulation of Estuarine Microbiota on Substrate Coated Agar Slides: A Novel Approach to Study Diversity of Autochthonous Bdellovibrio- and Like Organisms. Microbial Ecology. 55: 640-650 (2008).
Kit Components
| Component | 12400-50 | 12400-100 | 12400-250 |
|---|---|---|---|
| GelBIND | 1 x 33 ml | 1 x 63 ml | 1 x 158 ml |
| GelWASH | 1 x 16.5 ml | 1 x 30 ml | 3 x 30 ml |
| Elution Buffer | 1 x 3.3 ml | 1 x 6 ml | 1 x 15 ml |
| Collection Tubes | 50 | 100 | 250 |
| Spin Filters In Tubes | 50 | 100 | 250 |
(Mini Horizontal Gel System (cat # 16001) not included with this kit)
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