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UltraClean® GelSpin® DNA Extraction Kit
Overview
For fast and easy 5 minute DNA extraction from gels.ON SALE NOW! (Sale price is valid through 6/30/13 for US direct web orders only)
Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 12400-50 |
$87.00
$70.00 You Save: $17.00 N/A |
|
| 100 preps | 12400-100 |
$152.00
$120.00 You Save: $32.00 N/A |
|
| 250 preps | 12400-250 |
$363.00
$290.00 You Save: $73.00 N/A |
|
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Features
- Purifies DNA from agarose gels in as little as 5 minutes.
- Utilizes a simple method to remove ethidium bromide, linkers and labels from TAE or TBE gels.
- Simply melt, bind, wash and collect DNA.
Description
20 years later, MO BIO's first kit is still the fastest way to extract DNA from agarose gels! Help MO BIO celebrate our 20th Anniversary with 20% off your purchase of any size UltraClean® GelSpin® DNA Extraction Kit through June 30, 2013.
The UltraClean® GelSpin® DNA Extraction Kit utilizes a silica-based spin filter membrane to isolate DNA from agarose gels. After electrophoresis, the desired DNA band is cut from the agarose gel and placed directly in the spin filter column. The kit uses a very simple technique to melt the gel, bind the DNA, wash it and recover it. Popular uses include purification from gels, restriction digests, sequencing reactions (dye removal), radioactive and non-radioactive labeling reactions. The kit also removes all gel stains like ethidium bromide, SYBR® Green and xylene cyanol. The DNA is immediately ready to use for restriction digest, ligation, labeling or PCR.
Sample Kit
Click to try a sample.
Research and Development
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 |
from a 100 bp DNA ladder (lane 13) with the
UltraClean® GelSpin® DNA Extraction Kit.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| DNA Size Range | 100 bp - 50 kb |
| Binding Capacity | 20 µg DNA |
| Throughput | 1 - 24 samples |
| Time | 5 minutes |
| Size vs. Recovery | |
|---|---|
| DNA Size | Recovery |
| 60 bp 100 bp 300 bp 500 bp 10 kb 50 kb |
75% 80% 100% 95% 90% 80% |
Storage Conditions
Store at room temperature for up to 2 years.
Publications
- Michael S. Robeson, Andrew J. King, Kristen R. Freeman, C. William Birky, Jr, Andrew P. Martin, and Steven K. Schmidt. Soil rotifer communities are extremely diverse globally but spatially autocorrelated locally. PNAS, Mar 2011; 108: 4406 - 4410.
- Liis Uusküla, Kristiina Rull, Liina Nagirnaja, and Maris Laan. Methylation Allelic Polymorphism (MAP) in Chorionic Gonadotropin β5 (CGB5) and Its Association with Pregnancy Success. J. Clin. Endocrinol. Metab., Jan 2011; 96: E199 - E207.
- James J. Polashock, Rajeev Arora, Yanhui Peng, Dhananjay Naik, and Lisa J. Rowland. Functional Identification of a C-repeat Binding Factor Tnaldc.nal.usda.gov/catalog/41923ranscriptional Activator from Blueberry Associated with Cold Acclimation and Freezing Tolerance J. Amer. Soc. Hort. Sci., Jan 2010; 135: 40 - 48.
Kit Components
| Component | 12400-50 | 12400-100 | 12400-250 |
|---|---|---|---|
| GelBIND | 33 ml | 63 ml | 158 ml |
| GelWASH | 16.5 ml | 30 ml | 3 x 30 ml |
| Elution Buffer | 3.3 ml | 6 ml | 15 ml |
| Collection Tubes | 50 | 100 | 250 |
| Spin Filters In Tubes | 50 | 100 | 250 |
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