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UltraClean® GelSpin® DNA Extraction Kit

Overview

For fast and easy 5 minute DNA extraction from gels.
Order
Description Catalog # Price
50 preps 12400-50 $89.00    
100 preps 12400-100 $156.00    
250 preps 12400-250 $371.00    

Features

  • Purifies DNA from agarose gels in as little as 5 minutes
  • Utilizes a simple method to remove ethidium bromide, linkers and labels from TAE or TBE gels
  • Simply melt, bind, wash and collect DNA

Description

The UltraClean® GelSpin® DNA Extraction Kit utilizes a silica-based spin filter membrane to isolate DNA from agarose gels. After electrophoresis, the desired DNA band is cut from the agarose gel and placed directly in the spin filter column. The kit uses a very simple technique to melt the gel, bind the DNA, wash it and recover it. Popular uses include purification from gels, restriction digests, sequencing reactions (dye removal) and radioactive as well as non-radioactive labeling reactions. The kit also removes all gel stains like ethidium bromide, SYBR® Green and xylene cyanol. The DNA is immediately ready to use for restriction digest, ligation, labeling or PCR.

Sample Kit

Click to try a sample.

Research and Development

 

 

1 2 3 4 5 6 7 8 9 10 11 12 13    

DNA fragments in lanes 1-12 were recovered
from a 100 bp DNA ladder (lane 13) with the
UltraClean® GelSpin® DNA Extraction Kit.

 

UltraClean® GelSpin®  DNA Extraction Kit
Click over image to enlarge

MSDS

12400

Protocol

12400

Specifications

Format Silica Spin Filter Tubes
DNA Size Range 100 bp - 50 kb
Binding Capacity 20 µg DNA
Throughput 1 - 24 samples
Time 5 minutes
Size vs. Recovery
DNA Size Recovery
60 bp
100 bp
300 bp
500 bp
10 kb
50 kb
75%
80%
100%
95%
90%
80%

Storage Conditions

Store at room temperature for up to 2 years.

Publications

Customer Comments

An inexpensive and reliable kit for DNA purification

"The UltraClean® GelSpin® DNA Purification Kit allows the recovery of small DNA fragments from 0.2 mg agarose slices. DNA of 100 bp-50kb can be recovered. We have mostly used the kit for gel purification of 200-500 bp DNA fragments, with very good results."...read the entire review at Biocompare.com

Kit Components

Component 12400-50 12400-100 12400-250
GelBIND 33 ml 63 ml 158 ml
GelWASH 16.5 ml 30 ml  3 x 30 ml
Elution Buffer 3.3 ml 6 ml 15 ml
Collection Tubes 50 100 250
Spin Filters In Tubes 50 100 250