PowerBiofilm™ DNA Isolation Kit

Overview

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Features

• Complete lysis: Pretreatment + mechanical lysis (bead beater or vortex) + chemical lysis

• Patented IRT inside: increased sensitivity in qPCR

• Consistent high yields: more successful preps for better performance in downstream applications

•  Compatible with most biofilms: from dental plaques to microbial mats

Description

The PowerBiofilm™ DNA Isolation Kit pairs a brand-new bead beating and cell lysis technology with our patented Inhibitor Removal Technology® (IRT) for increased yields of inhibitor free DNA  from all types of biofilms, including microbial mats.

The PowerBiofilm™ DNA Isolation Kits from MO BIO Laboratories, Inc., have been specifically designed to overcome inefficient cell lysis.  The key to the success of these kits is our new bead tube formulation that combines brand-new chemical and mechanical lysis in tough tubes. Additionally, these tubes allow flexibility in the bead beating method (vortex or bead beater). Next, we’ve paired this with our Inhibitor Removal Technology® (IRT) to remove the concentrated inhibitors (such as humic acid, metals, salts and pesticides) typically present in biofilms. 

For more information, visit www.mobio.com/biofilm.

*This product is for research use only

Sample Kit

Click to try a sample.

Research and Development

  Successful Isolation of DNA From Diverse Sample Types

 Robust PCR results from low DNA yielding stream epilithic biofilms

 DNA was extracted with PowerBiofilm™ DNA Isolation Kit from stream epilithic biofilms. Stream pebbles were subjected to sonication to remove biofilm, pelleted with centrifugation then resuspended in Buffer BF1 and transferred to the bead tube. 

Data provided by University of Stirling, UK: A.T. Gildemeister 

  Successful End-point PCR Results

The Kapa 2G Fast HotStart kit was used for PCR.  Primers used were universal for the 16S rRNA gene (StreF/StreR) and generated a 1,243 bp product. 

MSDS

 24000

Protocol

24000 

Specifications

View the full PowerBiofilm^™ Application Note

PowerBiofilm Trailer

Storage Conditions

Publications

• Mountfort, D.; Smith, K. F.; Kirs, M.; Kuhajek, J.; Adamson, J. E.; Institute, S. A. W. C.; Bag, P.; Nelson; Zealand, N. Development of single and multispecies detection methods for the surveillance and monitoring of marine pests in New Zealand. Aquatic Invasions 2012 Vol. 7 No. 1 pp. 125-128

Kit Components