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BiOstic® Stabilized Blood RNA Isolation Kit
Overview
The BiOstic® Stabilized Blood RNA Isolation Kit provides a way to purify RNA from blood samples collected in PAXgene™ Blood RNA Tubes*.Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 20 preps | 12231-20 | $147.00 |
|
| 50 preps | 12231-50 | $347.00 |
|
| 100 preps | 12231-100 | $656.00 |
|
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Features
-Greater RNA yield compared to other PAXgene™ blood RNA tube extraction methods
-Enhanced mechanical lysis using our novel silica carbide DNA shearing column breaks down viscosity and releases more RNA for binding
-Proprietary cell pellet wash buffer ensures recovery and integrity of cells before extraction
-Available in 20, 50 or 100 prep sizes
-Complete and ready-to-use kit includes DNase I and prepared wash buffer for more consistent results
Description
The BiOstic® Stabilized Blood RNA Isolation Kit is designed for high yield isolation of total RNA from whole blood collected in PAXgene™ Blood RNA Tubes*. A ready-to-use and specially formulated was buffer ensures reliable and complete collection of cells at the start of the protocol and a novel silica carbide DNA shearing column provides more complete RNA solubilization and release for increased binding. Due to these improvements, the BiOstic® Stabilized Blood RNA Isolation Kit will purify total RNA from 2.5ml of stabilized blood with higher yields as compared to competitor kits (e.g. Competitor Q PAXgene™ Blood RNA kit).
The BiOstic® Stabilized Blood RNA Isolation Kit is sold for research use only.
*Kit does not include PAXgene™ tubes. PAXgene™ is a registered trademark of Qiagen
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Enzymatic and bead homogenization |
| Sample Size | One PAXgene™ Blood RNA Tube |
| Binding Capacity | Up to 20 µg per spin filter |
| Time | 65 minutes |
| Equipment Required | Vortex, centrifuge, and water bath or heat block |
Storage Conditions
Remove lyophilized DNase I and store at 4ºC. Proteinase K is stable at room temperature. For prolonged storage, place the Proteinase K at 4ºC. Store all other reagents and kit components at room temperature (15-30°C).
Note: After resuspension, store DNase I at -20°C (DNase I is sensitive to physical denaturation. Do not vortex the resuspended DNase I).
Kit Components
| Component | 12231-50 |
|---|---|
|
Solution PX1 Solution PX2 Solution PX3 Solution PX4 Solution PX5 Solution PX6 Solution PX7 Solution PX8 DNase I (RNase-Free) Proteinase K Solution Carbide Bead Tubes Spin Filters 2 ml Collection Tubes DNA Shearing Columns |
220 ml 20 ml 20 ml 20 ml 46 ml 3 ml 2 x 28 ml 6 ml 1500 units 2.25 ml 50 50 150 50 |
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