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Home / RNA Isolation / Blood RNA Isolation
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BiOstic® Blood Total RNA Isolation Kit

Overview

Isolate total RNA from up to 2 ml of whole blood, buffy coat cells, bone marrow, or up to 10 million cells.
Order
Description Catalog # Price
50 preps 12230-50 $275.00    

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Features

- Purify RNA from up to 2 ml of whole blood or 10 millions cells

- Fast and easy format with novel MO BIO flat bottom spin columns

- DNase 1 (Rnase-Free) included

- Isolate RNA from whole blood in 45 minutes

Description

The BiOstic® Blood Total RNA Isolation Kit provides a way to purify RNA from up to 2 ml of whole blood or 10 millions cells using a fast and easy spin column.
This method uses a simple hypotonic red blood cell (RBC) lysis to obtain a white blood cell (WBC) pellet for extraction of RNA.

Silica Spin column products utilize the novel MO BIO flat bottom spin column design, which provides improved samples processing and yields. The bucket configuration allows for enhanced samples flow and membrane drying after wash step since the entire membrane is accessible for air flows.

The silica technology provides a robust and fast way to purify nucleic acids without the use of organic solvents or cesium chloride gradients.

Sample Kit

Click here to try a sample.

Research and Development

 



Figure 1: RNA yields were twice as high compared to Qiagen using the BiOstic® Blood Total RNA Isolation Kit. Lane 1: Ladder; lane 2: MO BIO, avg. yield of 2.5 µg; lane 3: Qiagen, avg. yield 1.2 µg; lane 4: Invitrogen, avg. yield 1.6 µg



 



Figure 2: Successful RT-PCR with the BiOstic® Blood Total RNA Isolation Kit. RNA isolated is ready to use for enzymatic applications.
BiOstic® Blood Total RNA Isolation Kit
Click over image to enlarge

MSDS

12230

Protocol

12230

Specifications

Competition Spec MO BIO Supplier Q Supplier I

Starting volume of blood:

up to

 2 ml 1.5 ml 500 µl
Protocol speed 45 minutes 60 minutes 53 minutes
Dnase included? YES NO NO
Ready to use? YES NO NO
Average Yield (Fig. 1) 2.5 µg 1.2 µg 1.6 µg
Purity 260/280 2.02 1.92 1.62
Purity 260/230 1.61 1.14 0.65

 

Storage Conditions

Store RNase at 4°C. Store all other components at room temperature.

Kit Components

Component 12230-50 12230-20
Solution BR1
Solution BR2
Solution BR3
Solution BR4
Solution BR5
Solution BR6
Solution BR7
DNase 1
Spin Filters
2 ml Collection Tubes
15 ml Collection Tubes		 1 x 550 ml
1 x 40 ml
2 x 20 ml
2 x 23 ml
1 x 2.5 ml
2 x 28 ml
1 x 8 ml
1500 units
50
150
50		 1 x 220 ml
1 x 16 ml
1 x 16 ml
1 x 18 ml
1 x 1 ml
1 x 22 m1
1 x 3 ml
750 units
20
60
20