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UltraClean® -htp 96 Well BloodSpin® DNA Isolation Kit
Overview
For high throughput isolation of DNA from bloodOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 4 x 96 preps | 12296-4 | $887.00 |
|
| 12 x 96 preps | 12296-12 | $2,396.00 |
|
Features
- Isolate DNA from 96 samples of whole blood, buffy coat or cultured cells in 45 minutes.
- Yields DNA greater than 50 kb in length. DNA is ready for PCR, restriction digests, and all downstream applications.
- All tubes and reagents required are included.
Description
The UltraClean®-htp 96 Well BloodSpin® DNA Isolation Kit is designed to purify genomic and mitochondrial DNA from whole blood (fresh, frozen or stored at 4°C), buffy coat or cultured cells in 96 well plates. The protocol is designed as a centrifugation method.
In the presence of Proteinase K, cells are lysed, and proteins are degraded. The lysate is centrifuged through a silica membrane spin filter plate. DNA binds to the spin filter plate due to the high salt nature of the lysis buffer. The filter is washed and the DNA is recovered in certified DNA-free Tris buffer.
Research and Development
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| Lane 1: Marker Lanes 2-9: Undigested DNA |
Genomic DNA was isolated from whole blood using the UltraClean®-htp 96 Well Bloodspin® Kit and displayed on 1.2% TAE agarose gel.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | 96 Well Plate |
|---|---|
| Method | Silica Spin Plate |
| Starting Amount | 200 µl |
| Binding Capacity | Up to 40 µg per well |
| Throughput | 96 Samples |
| Time | 20 minutes |
| Equipment Required | Plate centrifuge |
Storage Conditions
New liquid Proteinase K is now stable at room temperature. All buffers and reagents can be stored at room temperature for up to 2 years.
Kit Components
| Component | 12296-4 | 12296-12 |
|---|---|---|
| Solution B1 Solution B2 Solution B3 Solution B4 Solution B5 Proteinase K Block Sealing Mat Centrifuge Tape Elution Sealing Mat Microplate Spin Plate Collection Plate |
1 x 85 ml 1 x 0 ml 1 x 93.3 ml 1 x 106 ml 1 x 85 ml 4.2 ml 8 16 4 4 4 12 |
1 x 255 ml 1 x 125 ml 1 x 279.9 ml 1 x 318 ml 1 x 255 ml 12.6 ml 24 48 12 12 12 36 |
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