Products by Application
Products by Category
Genomic DNA Isolation
Dye Dots
DNA Clean-Up
Plasmid DNA Isolation
RNA Isolation
DNA-Free Reagents
Homogenization Equipment
Growth Media
Plastics & Spin Filters
Lab Supplies
Certified Water
Enzymes
SamplesProduct Finder
Product Finder:Search for the best MO BIO
product for your application
UltraClean® BloodSpin® DNA Isolation Kit
Overview
Fast spin column protocol for isolating DNA from 200 µl of whole blood.Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 12200-50 | $112.00 |
|
| 250 preps | 12200-250 | $494.00 |
|
Related Products
UltraClean® PCR Clean-Up KitUltraClean® -htp 96 Well BloodSpin® DNA Isolation Kit
BiOstic® Bacteremia DNA Isolation Kit
UltraClean® Blood DNA Isolation Kit (Non-Spin)
Features
- Rapidly isolate DNA from whole blood, buffy coat or cultured cells in 20 minutes.
- Yields high quality DNA up to 50 kb ready for PCR and restriction digest.
- All tubes and reagent required are included (i.e. Proteinase K)
- Use UltraClean® BloodSpin® Kit for 200 µl whole blood.
- The UltraClean® BloodSpin® DNA Isolation Kit is also available in a 96 well plate format as the UltraClean®-htp 96 Well BloodSpin® DNA Isolation Kit.
Description
The UltraClean® BloodSpin® DNA Isolation Kit is designed to isolate genomic and mitochondrial DNA from whole blood (fresh, frozen or stored at 4°C), buffy coat or cultured cells.
In the presence of Proteinase K, cells are lysed, and proteins are degraded. The lysate is centrifuged through a silica membrane spin filter. DNA is bound to the spin filter due to the high salt nature of the lysis buffer. The filter is washed. Finally, the DNA is recovered in certified DNA-free Tris buffer.
Sample Kit
Click to try a sample.
Research and Development
|
|
-400bp | ||||||||||||||||
- Genomic DNA was isolated from whole blood using the UltraClean® Bloodspin® Kit and displayed on 1.2% TAE agarose gel (GEL A). PCR amplification was performed on isolated DNA using primers for human betaglobin (GEL B).
| Lane 1: Marker Lanes 2,3: Undigested DNA |
Lane 1: Marker Lane 2: PCR reaction |
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Starting Amount | 200 µl |
| Binding Capacity | Up to 40 µg per filter |
| Throughput | 1-24 Samples |
| Time | 20 minutes |
| Equipment Required | Microcentrifuge |
Storage Conditions
New liquid Proteinase K is now stable at room temperature. All buffers and reagents can be stored at room temperature for up to 2 years.Publications
- Queipo-Ortuño, M.I., Tena, F., Colmenero, J.D., Morata, P. Comparison Of Seven Commercial DNA Extraction Kits For The Recovery Of Brucella DNA From Spiked Human Serum Samples Using Real-Time PCR. European Journal of Clinical Microbiology & Infectious. 27(2): 109-114 (2008).
- Chang, J.H., Hampartzoumian, T, Everett, B., Lloyd, A., McCluskey, P.J., Wakefield, D. Changes In Toll-Like Receptor (TLR)-2 and TLR4 Expression and Function But Not Polymorphisms Are Associated With Acute Anterior Uveitis. Investigative Ophthalmology & Visual Science. 48(4): 1711-1717 (2007).
- Valiante, S., Prisco, M., Capaldo, A., Zambrano, I., De Falco, M., Andreuccetti, P., Laforgia, V., Varano, L. Molecular Characterization and Gene Expression Of The Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) In The Lizard Brain. Brain Research. 1127: 66-75.(2007).
Kit Components
| Component | 12200-50 | 12200-250 |
|---|---|---|
|
Solution B1 Solution B2 Solution B3 Solution B4 Solution B5 Proteinase K Spin Filters In Tubes Collection Tubes |
1 x 11 ml 1 x 11 ml 1 x 27.5 ml 1 x 30 ml 1 x 11 ml 1 x 550 µl 50 150 |
1 x 55 ml 1 x 55 ml 1 x 137.5 ml 1 x 160 ml 1 x 55 ml 1 x 2.75 ml 250 750 |
Related Products
|
For DNA clean up from PCR, sequencing and enzyme reactions.
|
|
||||||||||||||||
|
For high throughput isolation of DNA from blood
|
|
||||||||||||||||
|
*New* Isolate bacterial DNA from cultured blood
|
|
||||||||||||||||
|
For isolating high molecular weight DNA from whole blood without spin filters
|
|
||||||||||||||||