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UltraClean® Blood DNA Isolation Kit (Non-Spin)
Overview
For isolating high molecular weight DNA from whole blood without spin filtersOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 100 preps | 12000-100 | $96.00 |
|
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Features
- Yields high molecular weight DNA, over 100kb, that is ready for PCR, restriction digests and all downstream applications
- Gentle method eliminates DNA shearing
- Non-organic method avoids toxic chemicals, phenol and organic chemicals
- Removes PCR inhibitors and other contaminants. Purifies archival-quality DNA
- RNase A included
Description
The UltraClean® Blood DNA Isolation Kit (Non-Spin) is designed to isolate very high molecular weight genomic DNA from whole blood, buffy coat or cultured cells. It is a solution-based kit that employs a gentle procedure to eliminate shearing.
Lysis buffer is added directly to whole blood (fresh, frozen or stored at 4ºC). Next, cell nuclei and mitochondria are pelleted by centrifugation. The pellet is resuspended and incubated in a detergent solution, salt solution and RNase A to efficiently remove contaminants such as proteins and RNA. DNA is then precipitated by addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, dried and resupended in hydration buffer.
This UltraClean® Blood DNA Isolation Kit provides all the reagents and protocols for isolating high quality genomic, mitochondrial or viral (if associated with white blood cells) DNA from 300µl, 3 ml and up to 10ml of mammalian whole blood, buffy coat, bone marrow cells, culture cells, buccal swabs.
Sample Kit
Click to try a sample.
Research and Development
| 1 | 2 | 3 | 4 |
| Lane 1: Marker |
| Lane 2: Undigested DNA |
| Lane 3: Hae III digest |
| Lane 4: EcoR I digest |
- Genomic DNA was isolated from whole blood using the UltraClean® Blood DNA Isolation Kit (Non-Spin) and displayed on 1.2% TAE agarose gel.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Solution Based |
|---|---|
| Starting Amount | 300 µl |
| Binding Capacity | Up to 40 µg per prep |
| Throughput | 1-24 samples |
| Time | 1 hour |
| Equipment Required | Microcentrifuge |
Storage Conditions
Store at room temperature for up to 2 years.
RNase A Solution should be stored at 4°C for up to 2 years.
Kit Components
| Component | 12000-100 |
|---|---|
| Solution G1 Solution G2 Solution G3 Solution G4 Isopropanol 70% Ethanol RNase A Collection Tubes |
1 x 99 ml 1 x 33 ml 1 x 11 ml 1 x 11 ml 1 x 33 ml 1 x 30 ml 1 x 165 μl 200 |
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