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UltraClean® Blood DNA Isolation Kit(1,000 ml)+RNase
Overview
For isolating genomic DNA from 300 µl, 3 ml and up to 10 ml of whole blood. This kit yields archival-quality genomic DNA ready for any research application.Order
| Description | Catalog # | Price | |
|---|---|---|---|
| Processes up to 1,000ml + RNase | 12002-1000 | $1,058.00 |
|
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UltraClean® Blood DNA Isolation Kit (1,000 ml)UltraClean® Lab Cleaner
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Features
- Isolate DNA from whole blood, buffy coat, bone marrow or cultured cells in 20 minutes
- RNase A included
- Protocols are completely scalable for processing a variety of sample sizes up from 300µl, 3ml and up to 10ml
- Isolates high molecular-weight DNA from 100 to 200kb. DNA is ready for PCR, restriction digests and all downstream applications
- Contains no toxic chemicals, phenol or organic chemicals
- Removes PCR inhibitors and other contaminants. Purifies archival quality DNA.
Description
This UltraClean® Blood DNA Isolation Kit provides all the reagents and protocols for isolating high quality genomic, mitochondrial or viral (if associated with white blood cells) DNA from 300 µl, 3 ml and up to 10 ml of mammalian whole blood, buffy coat, bone marrow cells, culture cells, buccal swabs.
The DNA is ready for restriction digestion, PCR amplification, Southern hybridization, DNA sequencing, cloning and can be archived for years.
The UltraClean® Blood DNA Isolation Kit is designed to isolate high molecular weight genomic DNA from whole blood, buffy coat, bone marrow, cultured cells and other body fluids. It is a solution based kit that does not require spin filters and as a result, it is possible to isolate high molecular weight DNA, greater than 100 kb.
When isolating DNA from whole blood or bone marrow, the red blood cells, which lack genomic DNA, are first lysed to be separated from the leukocytes using RBC lysis buffer.
DNA is isolated from cells such as leukocytes, cells contained in body fluid or animal tissue by lysing the cells with an anionic detergent as a first step. An optional RNase A treatment can be used to efficiently remove any contaminating RNA. Cellular debris and proteins are precipitated using salt solution. DNA is finally precipitated by the addition of alcohol, washed in 70% ethanol, and resuspended in hydration buffer. This hydration buffer can be used to archive DNA for indefinite periods when stored at -80°C.
Sample Kit
Click to try a sample.
Research and Development
Lane 1: Marker; lane 2: Eco RI;
Lane 3: Hae III; Lane 4: Hind III.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Solution Based |
|---|---|
| Starting Amount | 300 µl, 3 ml and up to 10 ml |
| Yield |
50 - 150 µg (3 ml prep) |
| Throughput | 1-24 samples |
| Time | 1 hour |
| Equipment Required | Microcentrifuge |
Storage Conditions
Store at room temperature for up to 2 years.
RNase A Solution should be stored at 4°C for up to 2 years.
Kit Components
| Component | 12002-1000 |
|---|---|
| Solution G1 Solution G2 Solution G3 Solution G4 RNase A |
1 x 3030 ml 1 x 1010 ml 1 x 410 ml 1 x 70 ml 1 x 25 ml |
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