Archive for the ‘Tech Tips’ Category

0
comments

Water You Waiting For? Learn about working with water filters for isolation of DNA

May 01, 2013
Suzanne Kennedy

We speak with many scientists who work with filtered water for isolating microbial DNA and RNA. Water samples can be difficult because of their typically low biomass (depending on the water source) and because these samples are often from precious and unique sources.
Why is molecular research on microbes in water difficult?
For some people, getting back [...]

Read on...
2
comments

Where, Oh Where, Did My DNA Go…. a post on DNA Cleanup

Mar 27, 2013
Suzanne Kennedy

This week we want to discuss a technique that is very common but still causes many people to suffer from separation anxiety.  What could that possibly be? It is cleaning up dirty genomic DNA.
Here’s the scene: You have a precious soil sample collected from the roots of an ancient never-before seen orchid located on a [...]

Read on...
0
comments

Getting the RNA You Want

Feb 21, 2013
Michelle Carlson

We know many of our customers like to be selective about their RNA.  That’s because, most of our RNA technical questions involve a desire to retain or exclude certain varieties of RNA.   It’s not always possible to get what you want;  but sometimes by making slight adjustments to the extraction protocol, it is possible to [...]

Read on...
1
comment

The Scoop on Poop… Collection and Transport

Jan 18, 2013
Suzanne Kennedy

MO BIO is knee deep in questions about DNA and RNA isolation from stool thanks to some of our recently launched products, the PowerMicrobiome RNA Kit and PowerMag Microbiome RNA/DNA Isolation Kits.  One of the most frequently asked questions is how to store fecal samples for shipping and then storage for further processing.
I consulted with [...]

Read on...
2
comments

Like Water for Virus

Oct 22, 2012
Michelle Carlson

If you want to isolate microbial DNA from environmental water samples, you need to first separate the microbes from the water.   And since size exclusion filtering is one of the easiest methods to isolate microbes from large volumes of water, this is usually the preferred method.  With this in mind the MO BIO PowerWater DNA [...]

Read on...
0
comments

Filamentous Fungus Amongus!

Oct 10, 2012
Suzanne Kennedy

Our team came back from the 4th Annual Argonne Soil Metagenomics Conference held in the Chicago area October 3-5 last week and a hot topic of discussion was filamentous fungi.  Seems that ears were burning all over the cosmos as our technical support lines were also lighting up with questions about recommendations for isolation of [...]

Read on...
0
comments

Knock on Wood… and Isolate Fungal DNA!

Jun 08, 2012
Suzanne Kennedy

This weeks technical tip is a great question because, as we are all aware, no two environmental samples are ever quite alike. And when working with microbes, they exist anywhere and everywhere, so the substrate often complicates the matter. That’s why choosing a method for DNA isolation can be confusing. Luckily, here at MO [...]

Read on...
1
comment

What Bead Beating Instruments Work with the PowerSoil DNA Isolation Kit?

May 31, 2012
Suzanne Kennedy

A. Vortex
B. PowerLyzer
C. FastPrep
D. Precellys
E. All of the Above……
The answer is E! MO BIO Kits work with everything!
The tech tip for today is a great question!  Many people ask us about the adaptability of our kits with all the various bead beaters. Everything we make is compatible with any bead beater on the market. Read more…..
Hello,

Read on...
2
comments

How Dirty is DNA Without IRT?

May 24, 2012
Michelle Carlson

Scientists often come to us with their dirty little DNA problems. [...]

Read on...
5
comments

Technical Question of the Week: Can I Use Ethanol as a Preservative for Microbes?

May 17, 2012
Suzanne Kennedy

Welcome back readers! After a short break from blogging, we’re back with some new and different ideas to share with you. We’ll start off with a new weekly feature called Tech Tip Thursday (#MBTTT on twitter) where we share some of our customer’s questions and our answers with you. What better way of saving you [...]

Read on...