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Archive for the ‘New papers in microbiology’ Category

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Multiple Displacement Technology Goes Digital: a method for enumeration of nucleic acid contamination

During the ISME conference this past summer I had the opportunity to meet Richard White, and since then, we regularly converse about our research and improved techniques for some of his difficult and unusual samples. Rick is a graduate student in the lab of Curtis Suttle at the University of British Columbia studying microbial diversity in Pavilion [...]

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Next Generation Sequencing Data Analysis Made Easier with PANGEA

 Next generation sequencing is a powerful method increasing in popularity for use in metagenomic and transcriptomic analysis in environmental microbiology. Compared to Sanger sequencing, next generation allows for sequencing of the complete genomic content of a sample without the need to make clone libraries. Using this technique, microbial community analysis can be performed in a [...]

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Molecular Fingerprinting of the Gut Microbiota

One of Dr. Janet Jansson’s many ongoing projects, and a big focus in microbiology over the last few years has been the study of the human gut microbiome. As ASM 2010 draws near, you can be sure that human microbiome research is an area that will get a lot of attention.
I thought it would be a good time to review [...]

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Hot Off the Press: Current Research in Microbiology

In an ideal world, we would have time to read every great paper coming out in all the microbiology journals each month.  Instead we have to focus our reading on the topics that directly impact our research. But reading papers from other fields can often help us to find new methods or generate ideas for our projects [...]

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Journal Club: Identification of Pathogenic Amoebae in Warm Ground Water Aquifers

In a previous article, we reviewed a paper that investigated the microbial communities living in showerhead biofilms coming from the municipal water supply. This week, we’ll take another look at what’s living in municipal drinking water and this time in Phoenix, Arizona.  This new 2010 paper from the Journal of Environmental Quality takes a close look at the [...]

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Journal Club: Get Rid of False-Positives in 16s rDNA PCR

How many of you run into problems of false- positive PCR or lack of sensitivity in qPCR when trying to use 16S primers because of the background genomic DNA in your PCR enzyme mixes?
Well, then check out this paper by a team on Switzerland on how to remove residual contaminating genomic DNA from the enzyme [...]

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It’s Raining Bacteria….from the Showerhead!

How many of us dare to look up and actually take notice of the cleanliness of the showerhead during our morning shower? How often do you ever take a little Clorox to the showerhead?
Well according to a study published in PNAS this month (PNAS, Sep 2009; 106: 16393 – 16399) by Leah M. Feazel, Laura [...]

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