Comments on: Popular Misconceptions about DNA Isolation and Quantification http://www.mobio.com/blog/2010/01/31/popular-misconceptions-about-dna-isolation-and-quantification/ Tue, 13 Dec 2011 00:32:47 +0000 http://wordpress.org/?v=2.9.1 hourly 1 By: Determining the Best Homogenization Protocol for Any Soil « MO-BIO: The Culture Dish http://www.mobio.com/blog/2010/01/31/popular-misconceptions-about-dna-isolation-and-quantification/comment-page-1/#comment-8030 Determining the Best Homogenization Protocol for Any Soil « MO-BIO: The Culture Dish Sun, 30 Jan 2011 03:04:35 +0000 http://www.mobio.com/blog/?p=635#comment-8030 [...] There are many other reasons why a spec reading for DNA may not match the actual yield. I wrote about this in our very popular article Popular Misconceptions about DNA Isolation and Quantification. [...] [...] There are many other reasons why a spec reading for DNA may not match the actual yield. I wrote about this in our very popular article Popular Misconceptions about DNA Isolation and Quantification. [...]

]]> By: Suzanne Kennedy http://www.mobio.com/blog/2010/01/31/popular-misconceptions-about-dna-isolation-and-quantification/comment-page-1/#comment-7207 Suzanne Kennedy Sat, 23 Oct 2010 18:59:27 +0000 http://www.mobio.com/blog/?p=635#comment-7207 Hi Travers, Thanks for the comment on our blog. The best way to concentrate the DNA would be to ethanol precipitate it. We do have a product called UltraClean 15 that is a resin and you could try concentrating the DNA down to 10-20 ul with this as well. For water samples, if you are using a 47 mm water filter, we do have a different kit with a bead tube that fits the filter better. But if you don't want to switch kits, you can use the PowerSoil Kit but you'll need to use a high powered bead beater for the homogenization to make sure the bacteria are well lysed in that small tube. If you do not plan to filter it but want to start with a pellet, that is fine too. We have a few better kits for that as well (by better, I mean less steps and with bead tubes optimized for bacterial pellets), such as PowerFood, Bacteremia, and UltraClean Microbial DNA Kit. Hi Travers,
Thanks for the comment on our blog. The best way to concentrate the DNA would be to ethanol precipitate it. We do have a product called UltraClean 15 that is a resin and you could try concentrating the DNA down to 10-20 ul with this as well.
For water samples, if you are using a 47 mm water filter, we do have a different kit with a bead tube that fits the filter better. But if you don’t want to switch kits, you can use the PowerSoil Kit but you’ll need to use a high powered bead beater for the homogenization to make sure the bacteria are well lysed in that small tube.
If you do not plan to filter it but want to start with a pellet, that is fine too. We have a few better kits for that as well (by better, I mean less steps and with bead tubes optimized for bacterial pellets), such as PowerFood, Bacteremia, and UltraClean Microbial DNA Kit.

]]> By: Travers http://www.mobio.com/blog/2010/01/31/popular-misconceptions-about-dna-isolation-and-quantification/comment-page-1/#comment-7123 Travers Thu, 07 Oct 2010 00:55:29 +0000 http://www.mobio.com/blog/?p=635#comment-7123 That is very helpful information, especially the information on the expected yields. I have a question: if I have a soil sample that has a low number of bacteria, what is the best way to concentrate my sample to produce higher yields? Also, is it possible to use the Soil DNA extraction kit to extract DNA from, say, a water sample? (So that I don't have to go out and buy another kit) . I imagine I can spin down the sample and simply resuspend it in the bead tube, or is this not a good idea? That is very helpful information, especially the information on the expected yields.

I have a question: if I have a soil sample that has a low number of bacteria, what is the best way to concentrate my sample to produce higher yields?

Also, is it possible to use the Soil DNA extraction kit to extract DNA from, say, a water sample? (So that I don’t have to go out and buy another kit) . I imagine I can spin down the sample and simply resuspend it in the bead tube, or is this not a good idea?

]]> By: Tweets that mention Popular Misconceptions about DNA Isolation and Quantification « MO-BIO: The Culture Dish -- Topsy.com http://www.mobio.com/blog/2010/01/31/popular-misconceptions-about-dna-isolation-and-quantification/comment-page-1/#comment-385 Tweets that mention Popular Misconceptions about DNA Isolation and Quantification « MO-BIO: The Culture Dish -- Topsy.com Fri, 05 Feb 2010 16:00:11 +0000 http://www.mobio.com/blog/?p=635#comment-385 [...] This post was mentioned on Twitter by Dan Rhoads, Dan Rhoads and Nick Oswald, Suzanne Kennedy. Suzanne Kennedy said: @BitesizeBio Thanks Nick! The Culture Dish hit a record number of views today http://tinyurl.com/ydtkqqu. What's up next for Bitesizebio? [...] [...] This post was mentioned on Twitter by Dan Rhoads, Dan Rhoads and Nick Oswald, Suzanne Kennedy. Suzanne Kennedy said: @BitesizeBio Thanks Nick! The Culture Dish hit a record number of views today http://tinyurl.com/ydtkqqu. What's up next for Bitesizebio? [...]

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