Comments on: Journal Club: Get Rid of False-Positives in 16s rDNA PCR http://www.mobio.com/blog/2009/12/06/journal-club-get-rid-of-false-positives-in-16s-rdna-pcr/ Sun, 29 Aug 2010 00:08:49 +0000 http://wordpress.org/?v=2.9.1 hourly 1 By: A Decade Later, Human Gene Map Yields Few New Cures | DailyGenetic.info http://www.mobio.com/blog/2009/12/06/journal-club-get-rid-of-false-positives-in-16s-rdna-pcr/comment-page-1/#comment-2599 A Decade Later, Human Gene Map Yields Few New Cures | DailyGenetic.info Sun, 13 Jun 2010 19:45:40 +0000 http://www.mobio.com/blog/?p=556#comment-2599 [...] Journal Club: Get Rid of False-Positives in 16s rDNA PCR « MO-BIO … [...] [...] Journal Club: Get Rid of False-Positives in 16s rDNA PCR « MO-BIO … [...]

]]> By: Irag http://www.mobio.com/blog/2009/12/06/journal-club-get-rid-of-false-positives-in-16s-rdna-pcr/comment-page-1/#comment-1853 Irag Wed, 26 May 2010 14:58:14 +0000 http://www.mobio.com/blog/?p=556#comment-1853 Thanks Suzanne. I emailed the authors,and waiting for their reply. I think, it was a novel method. Thanks Suzanne. I emailed the authors,and waiting for their reply. I think, it was a novel method.

]]> By: Suzanne Kennedy http://www.mobio.com/blog/2009/12/06/journal-club-get-rid-of-false-positives-in-16s-rdna-pcr/comment-page-1/#comment-1852 Suzanne Kennedy Wed, 26 May 2010 14:23:36 +0000 http://www.mobio.com/blog/?p=556#comment-1852 Hi Irag, It looks like from the article that it worked well in the mastermix and I think they heat inactivated it. You'll want to get a copy of the paper and check the methods. I only summarized it here but the paper has a lot more details. Also, you can email the authors if you need. I emailed them to clarify some question I had before posting the article and they replied fast. Thanks!! Suzanne Hi Irag,
It looks like from the article that it worked well in the mastermix and I think they heat inactivated it. You’ll want to get a copy of the paper and check the methods. I only summarized it here but the paper has a lot more details. Also, you can email the authors if you need. I emailed them to clarify some question I had before posting the article and they replied fast.
Thanks!!
Suzanne

]]> By: irag http://www.mobio.com/blog/2009/12/06/journal-club-get-rid-of-false-positives-in-16s-rdna-pcr/comment-page-1/#comment-1851 irag Wed, 26 May 2010 14:20:35 +0000 http://www.mobio.com/blog/?p=556#comment-1851 Hello: thanks for posting this article. Do you know, if the exonuclease III works well in the mastermix. I mean, does the buffer system support the function of the enzyme ? Or does it need a different reaction ? Hello: thanks for posting this article. Do you know, if the exonuclease III works well in the mastermix. I mean, does the buffer system support the function of the enzyme ? Or does it need a different reaction ?

]]> By: Suzanne Kennedy http://www.mobio.com/blog/2009/12/06/journal-club-get-rid-of-false-positives-in-16s-rdna-pcr/comment-page-1/#comment-621 Suzanne Kennedy Sun, 07 Mar 2010 07:36:09 +0000 http://www.mobio.com/blog/?p=556#comment-621 Hi Yoram, We use PES membranes in 0.2 micron pore size. This works well for most samples. It really depends on the sample. See our blog on water filters for more information. Best, Suzanne Hi Yoram,
We use PES membranes in 0.2 micron pore size. This works well for most samples. It really depends on the sample. See our blog on water filters for more information.
Best,
Suzanne

]]> By: Yoram Gerchman http://www.mobio.com/blog/2009/12/06/journal-club-get-rid-of-false-positives-in-16s-rdna-pcr/comment-page-1/#comment-620 Yoram Gerchman Sun, 07 Mar 2010 06:37:14 +0000 http://www.mobio.com/blog/?p=556#comment-620 Greetings Whatman have seem to put Anodisc producing on hold. Do you know of a good replacement filter for low titer? Thanks Yoram Yoram Gerchman Biology Haifa University in Oranin Israel Greetings
Whatman have seem to put Anodisc producing on hold. Do you know of a good replacement filter for low titer?

Thanks
Yoram

Yoram Gerchman
Biology
Haifa University in Oranin
Israel

]]> By: Popular Misconceptions about DNA Isolation and Quantification « MO-BIO: The Culture Dish http://www.mobio.com/blog/2009/12/06/journal-club-get-rid-of-false-positives-in-16s-rdna-pcr/comment-page-1/#comment-368 Popular Misconceptions about DNA Isolation and Quantification « MO-BIO: The Culture Dish Mon, 01 Feb 2010 07:52:32 +0000 http://www.mobio.com/blog/?p=556#comment-368 [...] Journal Club: Get Rid of False-Positives in 16s rDNA PCR [...] [...] Journal Club: Get Rid of False-Positives in 16s rDNA PCR [...]

]]> By: Twitted by down2earthnews http://www.mobio.com/blog/2009/12/06/journal-club-get-rid-of-false-positives-in-16s-rdna-pcr/comment-page-1/#comment-164 Twitted by down2earthnews Wed, 09 Dec 2009 03:04:53 +0000 http://www.mobio.com/blog/?p=556#comment-164 [...] This post was Twitted by down2earthnews [...] [...] This post was Twitted by down2earthnews [...]

]]>