Comments on: Microbial Janitors: Enabling natural microbes to clean up uranium contamination http://www.mobio.com/blog/2009/11/29/microbial-janitors-enabling-natural-microbes-to-clean-up-uranium-contamination/ Tue, 13 Dec 2011 00:32:47 +0000 http://wordpress.org/?v=2.9.1 hourly 1 By: Suzanne Kennedy http://www.mobio.com/blog/2009/11/29/microbial-janitors-enabling-natural-microbes-to-clean-up-uranium-contamination/comment-page-1/#comment-6946 Suzanne Kennedy Wed, 22 Sep 2010 19:58:25 +0000 http://www.mobio.com/blog/?p=547#comment-6946 Hi Laxman, Thanks for your comment. I could not find info on uranium mines but have some references using PowerSoil on acid mine soil samples: Diversity of Dissimilatory Sulfite Reductase Genes (dsrAB) in a Salt Marsh Impacted by Long-Term Acid Mine Drainage John W. Moreau, Robert A. Zierenberg, and Jillian F. Banfield Appl. Envir. Microbiol., Jul 2010; 76: 4819 - 4828. Here are some references for Powermax Soil Kit and contaminated soils: Denitrifying Bacteria Isolated from Terrestrial Subsurface Sediments Exposed to Mixed-Waste Contamination Stefan J. Green, Om Prakash, Thomas M. Gihring, Denise M. Akob, Puja Jasrotia, Philip M. Jardine, David B. Watson, Steven D. Brown, Anthony V. Palumbo, and Joel E. Kostka Appl. Envir. Microbiol., May 2010; 76: 3244 - 3254. Application of Molecular Techniques To Elucidate the Influence of Cellulosic Waste on the Bacterial Community Structure at a Simulated Low-Level-Radioactive-Waste Site Erin K. Field, Seth D'Imperio, Amber R. Miller, Michael R. VanEngelen, Robin Gerlach, Brady D. Lee, William A. Apel, and Brent M. Peyton Appl. Envir. Microbiol., May 2010; 76: 3106 - 3115. These papers should provide the information you are looking for. We offer samples of the PowerSoil and PowerMax kit so if you want to try them out, just let us know. Best, Suzanne Hi Laxman,
Thanks for your comment. I could not find info on uranium mines but have some references using PowerSoil on acid mine soil samples:
Diversity of Dissimilatory Sulfite Reductase Genes (dsrAB) in a Salt Marsh Impacted by Long-Term Acid Mine Drainage
John W. Moreau, Robert A. Zierenberg, and Jillian F. Banfield
Appl. Envir. Microbiol., Jul 2010; 76: 4819 – 4828.

Here are some references for Powermax Soil Kit and contaminated soils:

Denitrifying Bacteria Isolated from Terrestrial Subsurface Sediments Exposed to Mixed-Waste Contamination
Stefan J. Green, Om Prakash, Thomas M. Gihring, Denise M. Akob, Puja Jasrotia, Philip M. Jardine, David B. Watson, Steven D. Brown, Anthony V. Palumbo, and Joel E. Kostka
Appl. Envir. Microbiol., May 2010; 76: 3244 – 3254.

Application of Molecular Techniques To Elucidate the Influence of Cellulosic Waste on the Bacterial Community Structure at a Simulated Low-Level-Radioactive-Waste Site
Erin K. Field, Seth D’Imperio, Amber R. Miller, Michael R. VanEngelen, Robin Gerlach, Brady D. Lee, William A. Apel, and Brent M. Peyton
Appl. Envir. Microbiol., May 2010; 76: 3106 – 3115.

These papers should provide the information you are looking for. We offer samples of the PowerSoil and PowerMax kit so if you want to try them out, just let us know.
Best,
Suzanne

]]> By: Laxman Singh K http://www.mobio.com/blog/2009/11/29/microbial-janitors-enabling-natural-microbes-to-clean-up-uranium-contamination/comment-page-1/#comment-6917 Laxman Singh K Tue, 21 Sep 2010 10:38:35 +0000 http://www.mobio.com/blog/?p=547#comment-6917 Dear Sir/Madam, We required Environmental Soil DNA extraction kit which is suitable for Uranium mine tailing's/tailing soil and the extracted DNA should work for PCR amplification without any inhibitors, the soil/tailing(crushed powdery uranium ore/rock) sample collected from tailing ponds of Uranium mines which is having higher concentration of radioactive materials, heavy metal and other hazardous wastes. If your kit works for above mentioned tailing soil, Please may send a signed letter(soft copy) and also may enclose strong published papers on DNA extracted from Uranium affected/ mine tailings or soil if possible Laxman Sing laxman.bt.05@gmail.com, biotech.eptri@gmail.com Dear Sir/Madam,
We required Environmental Soil DNA extraction kit which is suitable for Uranium mine tailing’s/tailing soil and the extracted DNA should work for PCR amplification without any inhibitors, the soil/tailing(crushed powdery uranium ore/rock) sample collected from tailing ponds of Uranium mines which is having higher concentration of radioactive materials, heavy metal and other hazardous wastes.
If your kit works for above mentioned tailing soil, Please may send a signed letter(soft copy) and also may enclose strong published papers on DNA extracted from Uranium affected/ mine tailings or soil if possible

Laxman Sing
laxman.bt.05@gmail.com, biotech.eptri@gmail.com

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